Platelet Rich Plasma

“The human body has a remarkable ability to heal itself.   Platelet Rich Plasma (PRP) is a concentration of the body’s own regenerative power.  PRP is one of the most advanced, effective natural treatments for cellular rejuvenation that can be used safely anywhere on the body.” -Dr. Lisbeth Roy

The Power of Platelet-Rich Plasma

Regeneration of new tissue is accomplished by the natural stimulation of stem cells and a flood of growth factors to the treated area. Platelet-rich plasma (PRP) is concentrated platelets and bioactive proteins derived from the patient’s own blood.  Once activated, platelets release growth factors that stimulate cellular regeneration and accelerate tissue repair.

PRP was initially used in wound healing, dentistry, and in the treatment of sports injuries.  Over the recent years, the use of PRP has evolved to address aesthetic concerns, hair regrowth, and physiologic dysfunction causing sexual changes and impairment. PRP is one of the most advanced, effective natural treatments for cellular rejuvenation that can be safely used throughout the body.

How Does PRP Work?

A PRP treatment begins with a simple blood draw.  PRP is processed using an FDA-approved medical device that separates and harvests plasma, platelets, anti-inflammatory white blood cells, and bioactive proteins.  The process to create PRP takes under 10 minutes.

The PRP is collected in a syringe and is injected back into your tissues where it’s needed. The growth factors that are released upon injection stimulate stem cells to create new, youthful tissue in areas like the face, scalp, sex organs, muscles, and joints. It’s like turning back the cellular clock.  What was old and damaged is repaired and made new; the essence of regenerative medicine.  

The beauty of treatment with PRP is the use of your own body’s regenerative power.  There is no risk of reaction or rejection since your body readily accepts the regenerative signal as its own. PRP procedures are minimally invasive with very little discomfort and downtime.

Advantages of Using PRP

Platelet-rich plasma is autologous, meaning it comes from your body and given back to you.  Because PRP is extracted from your own blood, it alleviates the risk of an allergic reaction, rejection, or skin sensitivity. More than 60,000 procedures involving the injection of PRP into various parts of the body have been performed with no side effects or complications reported.

PRP therapy is swiftly becoming one of the most popular treatment methods to address a variety of health and cosmetic issues, including:


  • Wrinkled or sagging skin
  • Frown lines, crow’s feet
  • Acne
  • Scarring
  • Rosacea
  • Erectile dysfunction
  • Peyronie’s disease
  • Urinary incontinence
  • Sexual wellness
  • Vaginal Dryness
  • Pain with intercourse
  • Lichen Sclerosis
  • Hair Regrowth
  • Joint and Muscle Pain

Procedures Harnessing the Power of PRP:

  • Studio Lift
  • Vampire Facial
  • Vampire Facelift
  • Vampire Breast lift
  • O-Shot
  • Priapus Shot
  • Studio Scalp

Doctors Studio uses the most current and advanced PRP processing methods and equipment.  Dr. Roy is committed to evolving her regenerative practice to advance the use of PRP as common practice.  StudioPRP.com is a group of medical providers who join Dr. Roy in the commitment to best patient outcomes when using PRP.  Each one of the Studio PRP providers uses the Emcyte Pure PRP II system to process PRP.


I have been treating patients and training physicians to use PRP since 2010 and have used nearly all the PRP systems sold in the US today. I am thrilled to know that there is a recent Call for Standards by clinicians and scientists. 

This “Call for Standards” is a result of the confusion in the marketplace. The fact is, not all PRP is equal in effectively recruiting mesenchymal stem cells, initiating angiogenesis, or creating the maximum regenerative potential. Many of the devices being used today to satisfy consumer demands do not meet the scientific definition of “Platelet Rich Plasma” and do not provide a consistent robust enough PRP to accomplish the best patient outcomes. 

My commitment to you and to my patients is to stay well informed as the science advances and provides “best practice” information regarding the best PRP cellular components and concentrations. PRP Science will move with this science to offer the equipment necessary to meet the standards.

Cellular Components Of PRP:


Red blood cells:  not often desirable in PRP

RBC’s add to the PRP viscosity, which increases injection difficulty and reduces injection site tissue saturation, and tends to significantly increase post-injection flare. Physicians can improve the application experience for themselves and their patients by allowing smaller gauge needles. RBC’s in PRP has also been attributed to increase pain and inflammation at the injection site after application.

Neutrophils:  not always desirable in PRP

Neutrophils are the most abundant leukocyte and one of the first responders to migrate towards a site of injury or infection (chemotaxis). Neutrophils are also the hallmark of acute inflammation. The primary function of the neutrophil is to engulf and destroy foreign material through phagocytosis. Under normal circumstances, neutrophils are short-lived (1-2 days) and are cleared by tissue macrophages. In conditions where the neutrophils cannot be cleared, for a lack of macrophages, they undergo a process called necrosis resulting in the release of all of the intracellular contents. This causes the amplification and prolonging of the inflammatory response. This prolonged amplified inflammatory response potential is a concern.

A PRP containing Neutrophils may be helpful if used in a surgical application where fighting and preventing infection is a focus.  However, a high Monocyte concentration without pro-inflammatory Neutrophils has significant cytokine activity and antimicrobial performance.

More pro-inflammatory Less pro-inflammatory mediators
More catabolic (cell-damaging) growth factors More anabolic response

Monocytes: always desirable in PRP

Monocytes are non-inflammatory white blood cells that are the precursor to macrophages. Macrophages are important cells of the immune system formed to fight infection or engulf accumulating damaged or dead cells. They are the largest-sized lymphocyte and are the longest lasting.

Deliverable platelet numbers

Deliverable platelets are the actual volume of viable platelets contained in a PRP sample. A high volume of deliverable platelets enhances the volumetric activity of platelet growth factors and cytokines inactive tissue repair.

Platelet alpha granules contain various platelet growth factors that promote tissue repair along with platelet cytokines that provide the chemical stimulus needed to attract and direct reparative cells to injured tissue.

Optimal Concentration of Platelets for the best migration of Mesenchymal (hMSC) stem cells to the treatment area is between 5-10 times baseline concentration (1.25 x 106/μL.to 2.5 x 106/μL when the baseline platelet count is 250 x 103/μL). (Haynesworth et al, 2002 48th annual meeting of the orthopedic research society)

Optimal Concentration of Platelets for Angiogenesis is 1.5 x 106/μL (Giusti, E, et al, Identification of an Optimal Concentration of Platelet Gel for Promoting Angiogenesis in Human Endothelial Cells, Transfusion, 2009; 49:771-778)

Rughetti has found that platelet count higher than 2 x 106/μL shows reversion of regenerative process. (The angiogenic effects of platelet gel: Blood Transfusion 2008; 6: 12-17 DOI 10.2450/2008.0026-07)

“Thus according to the current scientific literature, the “sweet spot” for platelet concentration to provide optimal stem cell migration, angiogenesis, and tissue regeneration is 6-7 times baseline (1.5 x 106/μL.to 2.1 x 106/μL when patient baseline platelet count is 250 x 103/μL).“


The best PRP for the effectiveness of the P-Shot, O-Shot, and other sexual medicine techniques is one that is 6 times baseline concentration of platelets with deliverable platelet counts of 1.5 x 106/μL, very few RBC, very few Granulocytes and a high monocyte count. This is the PRP I choose for my patients. Do yourself a favor and don’t pay for anything less than the “best PRP”.


You can not fix a problem you don’t understand. Advanced diagnostics if often necessary to get at the root cause. Once the root cause is identified, the solution becomes possible.

Dr. Lisbeth Roy, D.O.

Founder of Doctors Studio

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